Part:BBa_K3007004:Design

Expression device of dsRed under control of uric acid-responsive regulatory system

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1399
  Illegal EcoRI site found at 3216
  Illegal XbaI site found at 3113
  Illegal SpeI site found at 917
  Illegal PstI site found at 140
  Illegal PstI site found at 510
  Illegal PstI site found at 3210
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1399
  Illegal EcoRI site found at 3216
  Illegal NheI site found at 1085
  Illegal SpeI site found at 917
  Illegal PstI site found at 140
  Illegal PstI site found at 510
  Illegal PstI site found at 3210
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1399
  Illegal EcoRI site found at 3216
  Illegal BglII site found at 852
  Illegal BamHI site found at 158
  Illegal BamHI site found at 2266
  Illegal BamHI site found at 3130
  Illegal BamHI site found at 3205
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1399
  Illegal EcoRI site found at 3216
  Illegal XbaI site found at 3113
  Illegal SpeI site found at 917
  Illegal PstI site found at 140
  Illegal PstI site found at 510
  Illegal PstI site found at 3210
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1399
  Illegal EcoRI site found at 3216
  Illegal XbaI site found at 3113
  Illegal SpeI site found at 917
  Illegal PstI site found at 140
  Illegal PstI site found at 510
  Illegal PstI site found at 3210
  Illegal AgeI site found at 2084
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Figure 1. Schematic cartoon of the DNA construct of BBa_K3007004

Same as BBa_K3007029.

Source

HucR and PhucR come from D. radiodurans, while the others come from E. coli.